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Image Search Results
Journal: Investigative Ophthalmology & Visual Science
Article Title: Self-complementary AAV Virus (scAAV) Safe and Long-term Gene Transfer in the Trabecular Meshwork of Living Rats and Monkeys
doi: 10.1167/iovs.09-3847
Figure Lengend Snippet: Heat maps of sets of genes expressed in HTM cells infected with scAAV2.GFP and untreated control cells. Primary HTM cells were infected with scAAV2.GFP (∼1000 VP/cell; scAAV2) and harvested at 7 days after injection. Untreated (UNT) cells grown in parallel were used as a control. RNA was hybridized to microarray chips and analyzed with gene expression software. Expression signals in each chip were compared to a median value. Change from the median is visually represented by a color assignment (scale at right). Each row represents the expression of a single gene, and each column contains the genes selected in each category. (A) Expression levels of the total number of genes altered more than twofold in scAAV2.GFP-infected cells. (B) Expression levels of oxidative stress genes generated from the ontology gene list. (C) Expression levels of immune response genes generated from the ontology gene list. (D) Expression levels of extracellular matrix (ECM) genes generated from the ontology gene list. Most of the altered genes in scAAV were downregulated from the UNT. Expression of genes in the selected gene lists was barely affected in the scAAV2.GFP-infected cells.
Article Snippet:
Techniques: Infection, Injection, Microarray, Expressing, Software, Generated